Author affiliations * Corresponding authors a Faculty of Pharmacy, The
University of Sydney, Sydney, NSW 2006, Australia b CSIRO Manufacturing, Research Way, Clayton, VIC 3168, Australia c Microbiology Department, Freeman Hospital, Newcastle upon Tyne, UK d Sunderland
Pharmacy School, University of Sunderland, Sunderland, UK e bioMérieux Inc., Durham, NC, USA In order to retard the rate of development of antibacterial resistance, the causative agent must be identified as rapidly as possible, so that directed patient treatment and/or contact precautions can be initiated. This review highlights the challenges associated with
the detection and identification of pathogenic bacteria, by providing an introduction to the techniques currently used, as well as newer techniques that are in development. Focusing on the chemical basis for these techniques, the review also provides a comparison of their advantages and disadvantages. Article informationDOI https://doi.org/10.1039/C6CS00693KArticle type Tutorial ReviewSubmitted 23 Sep 2016First published 23 Jun 2017Download CitationChem. Soc. Rev., 2017,46, 4818-4832 Permissions
 Methods for the detection and identification of pathogenic bacteria: past, present, and futureL. Váradi, J. L. Luo, D. E. Hibbs, J. D. Perry, R. J. Anderson, S. Orenga and P. W. Groundwater, Chem. Soc. Rev., 2017, 46, 4818 DOI: 10.1039/C6CS00693K To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page. If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given. If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page. Read more about how to correctly acknowledge RSC content. Social activitySpotlightAdvertisementsRead Online (Free) relies on page scans, which are not currently available to screen readers. To access this article, please contact JSTOR User Support . We'll provide a PDF copy for your screen reader. With a personal account, you can read up to 100 articles each month for free. Get StartedAlready have an account? Log in Monthly Plan
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journal article Current molecular techniques for the detection of microbial pathogensScience Progress (1933-) Vol. 90, No. 1 (2007) , pp. 29-50 (22 pages) Published By: Sage Publications, Ltd. https://www.jstor.org/stable/43423199 Read and download Log in through your school or library Alternate access options For independent researchers Read Online Read 100 articles/month free Subscribe to JPASS Unlimited reading + 10 downloads Purchase article $45.50 - Download now and later Abstract Traditionally the detection of microbial pathogens in clinical, environmental or food samples has commonly neededtheprelevation of cells by culture before the application of the detection strategy. This is done to increase cell number thereby overcoming problems associated with the sensitivity of classical detection strategies. However, culture—based methods have the disadvantages of taking longer, usually are more complex and require skilled personnel as well as not being able to detect viable but non cultivable microbial species. A number of molecular methods have been developed in the last 10 to 15 years to overcome these issues and to facilitate the rapid, accurate, sensitive and cost effective identification and enumeration of microorganisms which are designed to replace and/or support classical approaches to microbial detection. Amongst these new methods, ones based on the polymerase chain reaction and nucleic acid hybridization have been shown to be particularly suitable for this purpose. This review generally summarizes some of the current and emerging nucleic acid based molecular approaches for the detection, discrimination and quantification of microbes in environmental, food and clinical samples and includes reference to the recently developing areas of microfluidics and nanotechnology "Lab-on-achip". Journal Information Science Progress commissions world authorities to contribute articles on the most interesting, important and meaningful topics - ranging from cosmology to the environment, and publishes occasional issues on specific topics. Publisher Information SAGE Publications is an academic and professional publisher. We publish books, journals and software under the SAGE, Corwin Press, Paul Chapman Publishing, Pine Forge Press, SAGE Reference, SAGE Science and Scolari (US and Europe websites) imprints. Rights & Usage This item is part of a JSTOR Collection. What are the traditional basis of detection and identification of pathogenic microorganisms?Traditional approaches for microbial detection and identification include microbial cultivation, immunological (e.g., antibody-based) assays, and nucleic acid detection schemes—especially amplification methods such as PCR (Tang et al., 1997; Fredricks and Relman, 1999).
What are molecular methods for identification of microorganisms?The molecular techniques for identification of microbes are single gene sequencing (e.g., by sequencing ribosomal RNA encoding 16S, 23S, 18S, and ITS gene), multiple gene sequencing (housekeeping and pathogenic genes), and whole-genome sequencing.
Which method is used for the identification of microorganisms on the basis of molecular biology?Single polymerase chain reaction
This is a polymerase chain reaction (PCR) involving the use of a single primer set (which targets a specific gene) to detect an organism. The primer set can be designed for specific species and can detect the target organism in the presence of others.
How do you identify a pathogenic microorganism?Biochemical testing. The majority of clinical microbiology laboratories still rely on culture for the detection of most bacterial pathogens from clinical samples. Traditionally, culture is performed using general purpose agar-based media (e.g. blood agar) that will support the growth of a wide range of pathogens.
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